The study of the anti-inflammatory effects of Baneh gum (Pistacia atlantica Desf. or mastic resin) in an in vivo mouse model of carrageenan-induced paw edema

Mirzaei Nodoushan, Majid; Amrollahi Sharifabadi, Mohammad; Salimi Sabour, Ebrahim; Jalali Kondori, Bahman; Shahriary, Alireza; Ezami, Masoud; Hosseini, Seyed Morteza; Sharifi Olounabadi, Ahmad Reza; Esmaeili Gouvrchin Ghaleh, Hadi

doi:10.30491/tm.2024.434185.1679

The study of the anti-inflammatory effects of Baneh gum (Pistacia atlantica Desf. or mastic resin) in an in vivo mouse model of carrageenan-induced paw edema

Document Type : Original Article

Authors

Majid Mirzaei Nodoushan ¹

Mohammad Amrollahi Sharifabadi ²

Ebrahim Salimi Sabour ³

Bahman Jalali Kondori ⁴

Alireza Shahriary ⁵

Masoud Ezami ⁶

Seyed Morteza Hosseini ⁷

Ahmad Reza Sharifi Olounabadi ⁷

Hadi Esmaeili Gouvrchin Ghaleh ¹

¹ Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

² Department of Basic Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.

³ Department of Pharmacognosy and Traditional Pharmacy, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran.

⁴ Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, Iran

⁵ Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

⁶ Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

⁷ Medicine, Quran and Hadith Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

10.30491/tm.2024.434185.1679

Abstract

Introduction: Although several studies have highlighted the beneficial effects of Baneh gum extract (BGE), characterizing its anti-inflammatory effects remains an unmet need. The present study aimed to investigate the in vivo effects of the BGE on the inflammation induced by carrageenan in an in vivo mouse model.
Methods: Inflammation induced using carrageenan. The animal population included 50 BALB/c mice with an age range of 6-8 weeks and a weight of 25-30 grams. The animals were divided into 5 groups (n=10 in each group) including inflammation control group (no treatment), intraperitoneal injection group before inflammation (25µg/ml BGE injected intraperitoneally), oral administration group before inflammation (25µg/ml BGE orally administered), the group of intraperitoneal injection concurrently with the induction of inflammation (25µg/ml BGE injected intraperitoneally), the group of oral administration concurrently with inflammation (25µg/ml BGE orally administered). Seven hours after the induction of inflammation, animals euthanized and samples harvested to measure biomarkers including pro-inflammatory and anti-inflammatory cytokines, antioxidant enzyme, oxidative stress indices, and liver enzymes.
Results: Our findings showed that the BGE can significantly reduce the amount of inflammatory cytokines and oxidative stress compared to the control group. Moreover, BGE can augment the amount of antioxidant enzymes and anti-inflammatory cytokines.
Conclusions: Based on the results of this study, it is proposed that the BGE can be a beneficial natural product with anti-inflammatory properties requiring further clinical studies to evaluate its potential application in the treatment of diseases with underlying cause of inflammation.

Keywords

Antioxidant enzymes

BGE

Oxidative stress

Subjects