Background: The known early and late complications following splenectomy increase the tendency to preserve splenic tissue after splenic trauma. Objectives: This study was conducted to determine the safety and feasibility of auto-transplantation of splenic fragments in patients with severe splenic injury. Methods: Nineteen patients with severe splenic blunt trauma, who underwent total splenectomy and autotransplantation of splenic fragments at Besat hospital, Hamadan University of Medical Sciences, Iran during years 2015 and 2016, were enrolled in the study. Complete blood counts, blood smear for Howell-Jolly bodies, and phagocytic function tests were performed just before, at 3 months, and 6 months after surgery. Results: The means of red blood cell counts increased significantly at both 3 and 6 months after surgery (P = 0.01 and 0.049, respectively) and the means of hemoglobin, hematocrit, and the percentage of lymphocytes increased significantly at 3 months after surgery (P = 0.001, 0.046, and 0.01 respectively) while this increase was not significant 6 months after surgery (P = 0.52, 0.15, and 0.34, respectively). The Howell-Jolly bodies were present in 5 patients (26.3%) at 3 months after surgery, which was significantly reduced to 3 (15.8%), at 6 months after surgery (P = 0.042). The median of splenic phagocytic function significantly increased from 3 ± 1.2 hotspots (range = 2 to 6) at 3 months after surgery to 4 ± 1.4 (range = 2 to 8) at 6 months after surgery (P = 0.044). Conclusions: Auto-transplantation of splenic fragments may be feasible and safe and a suitable option for splenic tissue salvage in patients with severe splenic lesions, who require total splenectomy.