Background: The effectiveness of topical phenytoin on wound healing has been cited in several research papers. However, methodological flaws and inappropriate controls, as well as the absence of randomization and double blinding devalue most of them. Objectives: We attempted a more stringent assessment of topical phenytoin powder and its role in the enhancement of wound healing. Methods: 76 rats were assigned and divided into two groups: phenytoin-treated and normal saline-treated. Phenytoin and saline were applied on incised open wounds in both groups. The efficacy of phenytoin and normal saline applications was assessed via morphological and histological evaluation over a 4-week period. Results: The results showed considerable reeducation in epithelization in the phenytoin-treated group vs. the control group over the study period. Neovascularization and tensile strength were significantly higher in phenytoin-treated rats as well. There was an insignificant difference regarding wound contraction time. Conclusions: Phenytoin application promoted wound healing. The healing properties of topical phenytoin powder were better in wounds.